Immunofluorescence labeling of cell surface antigens in Dictyostelium

Detection of cell-surface antigens using antibody-conjugated fluorospheres (ACF): application for six-color immunofluorescence.

Antibody-conjugated fluorospheres (ACF) were used to phenotype murine leukocytes by flow cytometric analysis. Multicolor immunofluoresence (beyond simultaneous 4-color analysis) is limited by the availability of specific antibody-fluorochrome chrome conjugates and even further restricted by the spectral emission overlap of many of the fluorochromes when used in combination. Fluorospheres posses...

Expression of S and pre s2 Hepatitis B Surface Antigens in Mammalian Cos-7 Cell Line

Hepatitis B virus (HBV) is a serious global health problem. The development of a safe and effective vaccinewould help infection prevention. Previous hepatitis B vaccine production involved the isolation of the noninfectious particle from chronic HBV carriers. DNA recombinant technology has been used for vaccineproduction without having been contaminated with blood-born infectious ag...

Double-labeling immunofluorescence: an alternative to detect multiple antigens in tissue sections.

I would like to make a comment about the review article: Technical aspects of immunohistochemistry by J. A. Ramos-Vara published in the July 2005 volume of Veterinary Pathology. In the paragraph: Detection of multiple antigens in a tissue section, the author describes enzyme-based methods to detect two antigens in tissues. Application of sequential staining method (two primary antibodies raised...

Comparison of membrane immunofluorescence and cytotoxicity tests for detection of cell surface antigens of Marek's disease lymphoma-derived cell line.

Patterns of cell movement within the Dictyostelium slug revealed by cell type-specific, surface labeling of living cells.

There are cells scattered in the rear, prespore region of the Dictyostelium slug that share many of the properties of the prestalk cells and that are therefore called anterior-like cells (ALCs). By placing the gene encoding a cell surface protein under the control of an ALC-specific promoter and immunologically labeling the living cells, we analyze the movement of ALCs within the slug. There is...